              SEQUENCE LISTING                       

<110>  Pharmassist Ltd
 
<120>  Method for the quantification of PD-L1 expression


<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  3691
<212>  DNA
<213>  Homo sapiens (PD-L1 mRNA sequence (NM_014143))

<400>  1
ggcgcaacgc tgagcagctg gcgcgtcccg cgcggcccca gttctgcgca gcttcccgag       60

gctccgcacc agccgcgctt ctgtccgcct gcagggcatt ccagaaagat gaggatattt      120

gctgtcttta tattcatgac ctactggcat ttgctgaacg catttactgt cacggttccc      180

aaggacctat atgtggtaga gtatggtagc aatatgacaa ttgaatgcaa attcccagta      240

gaaaaacaat tagacctggc tgcactaatt gtctattggg aaatggagga taagaacatt      300

attcaatttg tgcatggaga ggaagacctg aaggttcagc atagtagcta cagacagagg      360

gcccggctgt tgaaggacca gctctccctg ggaaatgctg cacttcagat cacagatgtg      420

aaattgcagg atgcaggggt gtaccgctgc atgatcagct atggtggtgc cgactacaag      480

cgaattactg tgaaagtcaa tgccccatac aacaaaatca accaaagaat tttggttgtg      540

gatccagtca cctctgaaca tgaactgaca tgtcaggctg agggctaccc caaggccgaa      600

gtcatctgga caagcagtga ccatcaagtc ctgagtggta agaccaccac caccaattcc      660

aagagagagg agaagctttt caatgtgacc agcacactga gaatcaacac aacaactaat      720

gagattttct actgcacttt taggagatta gatcctgagg aaaaccatac agctgaattg      780

gtcatcccag aactacctct ggcacatcct ccaaatgaaa ggactcactt ggtaattctg      840

ggagccatct tattatgcct tggtgtagca ctgacattca tcttccgttt aagaaaaggg      900

agaatgatgg atgtgaaaaa atgtggcatc caagatacaa actcaaagaa gcaaagtgat      960

acacatttgg aggagacgta atccagcatt ggaacttctg atcttcaagc agggattctc     1020

aacctgtggt ttaggggttc atcggggctg agcgtgacaa gaggaaggaa tgggcccgtg     1080

ggatgcaggc aatgtgggac ttaaaaggcc caagcactga aaatggaacc tggcgaaagc     1140

agaggaggag aatgaagaaa gatggagtca aacagggagc ctggagggag accttgatac     1200

tttcaaatgc ctgaggggct catcgacgcc tgtgacaggg agaaaggata cttctgaaca     1260

aggagcctcc aagcaaatca tccattgctc atcctaggaa gacgggttga gaatccctaa     1320

tttgagggtc agttcctgca gaagtgccct ttgcctccac tcaatgcctc aatttgtttt     1380

ctgcatgact gagagtctca gtgttggaac gggacagtat ttatgtatga gtttttccta     1440

tttattttga gtctgtgagg tcttcttgtc atgtgagtgt ggttgtgaat gatttctttt     1500

gaagatatat tgtagtagat gttacaattt tgtcgccaaa ctaaacttgc tgcttaatga     1560

tttgctcaca tctagtaaaa catggagtat ttgtaaggtg cttggtctcc tctataacta     1620

caagtataca ttggaagcat aaagatcaaa ccgttggttg cataggatgt cacctttatt     1680

taacccatta atactctggt tgacctaatc ttattctcag acctcaagtg tctgtgcagt     1740

atctgttcca tttaaatatc agctttacaa ttatgtggta gcctacacac ataatctcat     1800

ttcatcgctg taaccaccct gttgtgataa ccactattat tttacccatc gtacagctga     1860

ggaagcaaac agattaagta acttgcccaa accagtaaat agcagacctc agactgccac     1920

ccactgtcct tttataatac aatttacagc tatattttac tttaagcaat tcttttattc     1980

aaaaaccatt tattaagtgc ccttgcaata tcaatcgctg tgccaggcat tgaatctaca     2040

gatgtgagca agacaaagta cctgtcctca aggagctcat agtataatga ggagattaac     2100

aagaaaatgt attattacaa tttagtccag tgtcatagca taaggatgat gcgaggggaa     2160

aacccgagca gtgttgccaa gaggaggaaa taggccaatg tggtctggga cggttggata     2220

tacttaaaca tcttaataat cagagtaatt ttcatttaca aagagaggtc ggtacttaaa     2280

ataaccctga aaaataacac tggaattcct tttctagcat tatatttatt cctgatttgc     2340

ctttgccata taatctaatg cttgtttata tagtgtctgg tattgtttaa cagttctgtc     2400

ttttctattt aaatgccact aaattttaaa ttcatacctt tccatgattc aaaattcaaa     2460

agatcccatg ggagatggtt ggaaaatctc cacttcatcc tccaagccat tcaagtttcc     2520

tttccagaag caactgctac tgcctttcat tcatatgttc ttctaaagat agtctacatt     2580

tggaaatgta tgttaaaagc acgtattttt aaaatttttt tcctaaatag taacacattg     2640

tatgtctgct gtgtactttg ctatttttat ttattttagt gtttcttata tagcagatgg     2700

aatgaatttg aagttcccag ggctgaggat ccatgccttc tttgtttcta agttatcttt     2760

cccatagctt ttcattatct ttcatatgat ccagtatatg ttaaatatgt cctacatata     2820

catttagaca accaccattt gttaagtatt tgctctagga cagagtttgg atttgtttat     2880

gtttgctcaa aaggagaccc atgggctctc cagggtgcac tgagtcaatc tagtcctaaa     2940

aagcaatctt attattaact ctgtatgaca gaatcatgtc tggaactttt gttttctgct     3000

ttctgtcaag tataaacttc actttgatgc tgtacttgca aaatcacatt ttctttctgg     3060

aaattccggc agtgtacctt gactgctagc taccctgtgc cagaaaagcc tcattcgttg     3120

tgcttgaacc cttgaatgcc accagctgtc atcactacac agccctccta agaggcttcc     3180

tggaggtttc gagattcaga tgccctggga gatcccagag tttcctttcc ctcttggcca     3240

tattctggtg tcaatgacaa ggagtacctt ggctttgcca catgtcaagg ctgaagaaac     3300

agtgtctcca acagagctcc ttgtgttatc tgtttgtaca tgtgcatttg tacagtaatt     3360

ggtgtgacag tgttctttgt gtgaattaca ggcaagaatt gtggctgagc aaggcacata     3420

gtctactcag tctattccta agtcctaact cctccttgtg gtgttggatt tgtaaggcac     3480

tttatccctt ttgtctcatg tttcatcgta aatggcatag gcagagatga tacctaattc     3540

tgcatttgat tgtcactttt tgtacctgca ttaatttaat aaaatattct tatttatttt     3600

gttacttggt acaccagcat gtccattttc ttgtttattt tgtgtttaat aaaatgttca     3660

gtttaacatc ccagtggaga aagttaaaaa a                                    3691


<210>  2
<211>  987
<212>  DNA
<213>  Homo sapiens (B2M mRNA sequence (NM004048))

<400>  2
aatataagtg gaggcgtcgc gctggcgggc attcctgaag ctgacagcat tcgggccgag       60

atgtctcgct ccgtggcctt agctgtgctc gcgctactct ctctttctgg cctggaggct      120

atccagcgta ctccaaagat tcaggtttac tcacgtcatc cagcagagaa tggaaagtca      180

aatttcctga attgctatgt gtctgggttt catccatccg acattgaagt tgacttactg      240

aagaatggag agagaattga aaaagtggag cattcagact tgtctttcag caaggactgg      300

tctttctatc tcttgtacta cactgaattc acccccactg aaaaagatga gtatgcctgc      360

cgtgtgaacc atgtgacttt gtcacagccc aagatagtta agtgggatcg agacatgtaa      420

gcagcatcat ggaggtttga agatgccgca tttggattgg atgaattcca aattctgctt      480

gcttgctttt taatattgat atgcttatac acttacactt tatgcacaaa atgtagggtt      540

ataataatgt taacatggac atgatcttct ttataattct actttgagtg ctgtctccat      600

gtttgatgta tctgagcagg ttgctccaca ggtagctcta ggagggctgg caacttagag      660

gtggggagca gagaattctc ttatccaaca tcaacatctt ggtcagattt gaactcttca      720

atctcttgca ctcaaagctt gttaagatag ttaagcgtgc ataagttaac ttccaattta      780

catactctgc ttagaatttg ggggaaaatt tagaaatata attgacagga ttattggaaa      840

tttgttataa tgaatgaaac attttgtcat ataagattca tatttacttc ttatacattt      900

gataaagtaa ggcatggttg tggttaatct ggtttatttt tgttccacaa gttaaataaa      960

tcataaaact tgatgtgtta tctctta                                          987


<210>  3
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward target primer 1 (PD-L1)

<400>  3
tcatcccaga a                                                            11


<210>  4
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward target primer 2 (PD-L1)

<400>  4
gctgaattgg tcatcccaga a                                                 21


<210>  5
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse target primer 1 (PD-L1)

<400>  5
cattctccct t                                                            11


<210>  6
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse target primer 2 (PD-L1)

<400>  6
tttcacatcc atcattctcc ctt                                               23


<210>  7
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target hydrolysis probe 1 (PD-L1)

<400>  7
gcacatcctc ca                                                           12


<210>  8
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target hydrolysis probe 2 (PD-L1)

<400>  8
acctctggca catcctccaa atgaaag                                           27


<210>  9
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward reference primer 1 (B2M)

<400>  9
gccgtgtgaa c                                                            11


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward reference primer 2 (B2M)

<400>  10
gcctgccgtg tgaaccatgt                                                   20


<210>  11
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse reference primer 1 (B2M)

<400>  11
cttcaaacct c                                                            11


<210>  12
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse reference primer 2 (B2M)

<400>  12
aaatgcggca tcttcaaacc tc                                                22


<210>  13
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reference hydrolysis probe 1 (B2M)

<400>  13
ctcgatccca c                                                            11


<210>  14
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reference hydrolysis probe 2 (B2M)

<400>  14
catgatgctg cttacatgtc tcgatcccac                                        30


